Characterization of interferon alpha of major histocompatibility complex class I in Punjab urial (Ovis vignei punjabiensis)

Authors: TANVEER HUSSAIN, MUNEEB MUSTHAFA, MASROOR ELLAHI BABAR, ATIYA YASMEEN, ASIF NADEEM, NISAR AHMAD, FAIZ MARIKAR

Abstract: We have characterized the endangered Punjab urial via its interferon alpha-A gene. DNA extraction was done from blood, followed by PCR and sequencing using a 3130xl genetic analyzer. Single nucleotide polymorphism and phylogenetic analyses were performed with BioEdit 7.0.9.0 and MEGA 6.1. The highly polymorphic nature revealed will lead to identifying molecular markers for breeding purposes.

Keywords: Punjab urial, interferon, polymorphic, phylogenetic tree, endangered, Pakistan

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