Histopathologic Alterations Associated with Syphacia sp. (Nematode) in the Intestine of Nesokia indica

Authors: NASIRA KHATOON, FATIMA MUJIB BILQEES, DURE SHAHWAR JAFFERY, A. G. RIZWANA

Abstract: The present work was undertaken to study the histopathological changes in the intestine of male rat Nesokia indica. Histopathological sections of infected intestine were prepared by fixing small pieces of intestine in buffer formalin for 24 h. The fixed material was washed with 70% alcohol and dehydrated and cleared for paraffin block making by the usual procedure. Sections of 5 to 7 µ thickness were used for the present study. E & H stained serial sections were mounted permanently in DPX. Sections were examined in detail and photomicrographs of selected sections were taken by Nikon (Optiphot-2) photomicroscope using Fuji colour film. Histopathological study of selected portions of the intestine revealed that nematode parasites severely affected the whole thickness of the intestinal wall. Pronounced changes included atrophy of the villi and underlying tissue as a common finding. Granulomatous tissue masses and granulation lesions were also obvious. Hypertrophy and hyperplasia of the glandular region were seen. Pedunculate edema forming a condition like sessile villous adenoma was an important finding.

Keywords: Syphacia sp., Nematode, Nesokia indica, intestine, Histopathology

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