Alterations of HSP70 gene expression in rainbow trout (Oncorhyncus mykiss) exposed to deltamethrin

Authors: MUHAMMED ATAMANALP, ORHAN ERDOĞAN

Abstract: The aim of the study was to assess the effects of deltamethrin, a synthetic pyrethroid pesticide and potential toxic pollutant to aquatic ecosystems, on the heat shock protein HSP70 gene expression in rainbow trout. Deltamethrin was applied at 0.3 and 0.6 \mug/L for 28 days under laboratory conditions. After the experiment, HSP70 was amplified for 20-40 PCR cycles for the control and deltamethrin-treated fish groups. While the quantitative mRNA level was low in the 40^t^h PCR cycle in the control, it was higher in the fish exposed to deltamethrin. The differences between the treated groups and the control group were statistically significant (P < 0.01), but the difference between the treatment groups was not.

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