Comparison of Polymerase Chain Reaction and Conventional Methods for the Diagnosis of Listeria monocytogenes in Stuffed Mussels

Authors: ERGÜN Ö. GÖKSOY, ŞÜKRÜ KIRKAN, OSMAN KAYA

Abstract: The aim of this study was to evaluate the role of stuffed mussel as a source of Listeria monocytogenes. Polymerase chain reaction is a rapid procedure with both sensitivity and specificity for quick detection and identification of L. monocytogenes. A total of 50 mussel samples were investigated for L. monocytogenes. L. monocytogenes was not identified by the conventional method. However, PCR amplification products demonstrated that 5 out of 50 samples showed positive reactions with L. monocytogenes. The PCR positive samples showed specific amplification at approximately 343 bp for L. monocytogenes.

Keywords: Listeria monocytogenes, stuffed mussel, conventional methods, PCR

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