The effect of osteosubstitution by platelet-rich autofibrin and hydroxyapatite ceramic with ß-tricalcium phosphate on biochemical parameters of blood in rabbits

Authors: SVITLANA SHEVCHENKO, MYKHAILO RUBLENKO

Abstract: The paper presents the results of a rabbit blood biochemical study after the substitution of simulated defects in spongy and compact bone tissue by platelet-rich fibrin and its combination with hydroxyapatite granules with ß-tricalcium phosphate. The aim of the work is to establish the biochemical features of reparative osteogenesis in the time of osteosubstitution by platelet-enriched fibrin and its combination with hydroxyapatite and ß-tricalcium phosphate. Bone defects (d = 3 mm) were modeled in rabbits from control and experimental groups (n = 12) under general anaesthesia. In the control group, a replacement was performed with autologous fibrin enriched with platelets (PRF), and in the experimental group, it was combined with hydroxyapatite ceramics. Content of biochemical substances was determined: soluble fibrin, the activity of antithrombin-III in blood plasma, nitric oxide (NO), haptoglobin, and markers of bone metabolism (bone isoenzyme alkaline phosphatase (BAP) and tartrate-resistant acid phosphatase (TrAP) in serum. Measurements were performed with a spectrophotometer Stat Fax 4500. It is established that the dynamics of changes in biochemical parameters reflect certain features of osteosubstitution by different materials. In particular, the inflammatory-resorptive stage of reparative osteogenesis in the period up to the 7th day is characterized by an increase in blood concentrations of NO, haptoglobin, and soluble fibrin. However, the intensity of the acute phase in the case of combined osteosubstitution is significantly lower. The next peak of NO concentration on the 14th day indicates early angiogenesis with combined osteosubstitution. The two-phase degree of osteoresorption at the level of TrAP is significantly lower than combined osteosubstitution, and the intensity of osteogenic processes at the level of BAP, on the contrary, earlier. Also used biochemical parameters can be prognostic criteria for the analysis of fracture consolidation in conditions of osteosubstitution with different materials.

Keywords: Platelets, bone markers, tartrate-resistant acid phosphatase, bone alkaline phosphatase isoenzyme

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