Serological and Virological Investigations of Bovine Viral Diarrhoea Virus (BVDV) Infection in Dairy Cattle Herds in Aydın Province

Authors: MEHMET TOLGA TAN, M. TANER KARAOĞLU, NURAL EROL, YAKUP YILDIRIM

Abstract: A total of 288 dairy cattle from 4 different closed barns were sampled in order to investigate the presence of bovine viral diarrhoea virus (BVDV) infection. Based on the data gathered from neutralisation peroxidase-linked antibody (NPLA) tests, which were conducted to detect BVDV antibodies, all of the barns monitored previously had the infection; the rates of seropositive animals were between 44% and 100%. Overall, the results obtained from Aydın province showed that 248 of the 288 dairy cows (86%) monitored were seropositive. BVDV antigen ELISA kits (Bio-X Diagnostics- Belgium) were used to detect BVDV antigen (NS3 protein) in blood samples. The cattle found to be positive in the first trial were subjected to subsequent sampling 45 days later. Fourteen animals (4.9%) that were BVDV Ag positive during both trials were diagnosed as persistently infected with the virus.

Keywords: BVDV, epidemiology, ELISA, persistent infection

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