Effects of ATP-MgCl_2 on Myocardial Ischemia-Reperfusion Injury: An In Vivo Experimental Study

Authors: ZÜLFİKAR KADİR SARITAŞ, NUSRET APAYDIN, FATMA ESER ÖZGENCİL, KAMURAN PAMUK, A. TULGA ULUS, AYŞEN AKSOYEK, UFUK TÜTÜN, ALİ İHSAN PARLAR, SALİH FEHMİ KATIRCIOĞLU

Abstract: We investigated the effect of ATP-MgCl_2 on myocardial hemodynamics and metabolism. The study included 12 mongrel dogs that were randomly divided into 2 groups: the control and the ATP-MgCl_2 (study) groups. Following a right thoracotomy, the left anterior descending artery was ligated for 15 min and then reperfusion was performed. After the ischemic period, cardiopulmonary bypass was initiated and the aorta was cross-clamped. ATP-MgCl_2 (70+70 mmol/l) 0.25 ml/kg per h was given to the study group at the start of the ischemic period. Hemodynamic and metabolic measurements were obtained at basal, and then after 60, 120, and 180 min of reperfusion Cardiac output values were significantly higher in the study group; at the end of the third hour of reperfusion they were 2370 ± 31 ml/min in the study group and 900 ± 45 ml/min in the control group (P < 0.05). Myocardial Oxygen Extraction (MOE) and Myocardial Lactate Extraction (MLE) values were significantly higher in the control group; at the end of the third hour of the reperfusion MOE values were 50 ± 2 ml in the study group and 55 ± 3 ml in the control group, while MLE values were -0.06 ± 0.02 mmol/ml in the study group and -0.12 ± 0.07 mmol/ml in the control group (P < 0.05). Hemodynamic and metabolic parameters revealed that the use of ATP-MgCl_2 might be beneficial in reducing ischemia-reperfusion damage.

Keywords: In vivo experimental study, ischemia-reperfusion injury, myocardium, ATP-MgCl_2

Full Text: PDF