Immune Responses in Mice Immunized Intranasally With Sendai Virus

Authors: GAZIM BIZANOV, IRENA JONAUSKIENE

Abstract: Mice were immunized 3 times intranasally (i.n.) at intervals of 2 weeks with a formalin-inactivated Sendai virus (SV) whole virus with and without whole formalin-inactivated Bordetella pertussis (Bp) as adjuvant. The antibody response to SV was measured using the hemagglutination inhibition (HI) test in serum, and the IgA and IgG antibodies were evaluated using enzyme linked immunosorbent assay in saliva and serum. It was demonstrated that repeating immunizations with SV plus Bp could induce HI serum antibodies, IgA and serum IgG antibodies. However, in mice immunized subcutaneously with SV alone, high serum IgG antibodies and HI titers were induced only. In contrast, the animals immunized i.n. with SV alone had significantly lower titers to SV than those of the other groups.

Keywords: Adjuvant, intranasal, Sendai virus, pertussis

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