Microsatellite panels for parentage testing of Kilis goats reared in Turkey

Authors: MAHMUT KESKİN, ONUR YILMAZ, ZÜHAL GÜNDÜZ, NEZİH ATA, SABRİ GÜL, İBRAHİM CEMAL, ORHAN KARACA, SÜLEYMAN ERCÜMENT ÖNEL

Abstract: The aim of this study was to develop PCR-based suitable microsatellite marker panels for paternity testing and to define pedigree errors in Kilis goats. A total of 137 head of goats were used, consisting of 118 head of kids and 19 head of possible candidate sires. A total of 392 alleles were observed in 22 microsatellite markers. Allele numbers ranged between 12 (SRCRSP7) and 24 (BM1818, INRA0023, and SRCRSP15) with an average of 17.82. The mean value of the effective allele numbers was obtained as 9.44. The overall polymorphic information content value was quite high (0.88). The overall observed (Ho) and expected heterozygosity (He) values for all studied loci were 0.89 and 0.89, respectively. Paternity test panels for the Kilis goat population studied were created based on individual probability of exclusion of microsatellites in multiplex groups. Combined probability of exclusion (CPE) values for different panels ranged between 0.745 (Panel-1) and 0.9999 (Panel-22), while the combined identification probability (CPI) values were obtained in the range of 9.81 × 10$^{-3}$ (Panel-1) to 6.96 × 10$^{-21}$ (Panel-22). As a result of this study, it can be stated that panels with 0.999 CPE values can be used at the most reasonable cost and with high reliability in paternity tests to be performed in Kilis goats, which can be a reference for other populations.

Keywords: Kilis goats, microsatellite, parentage testing, probability of exclusion

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