The Presence, Isolation and Characterisation of Galectin-1 in Bovine Adrenal Gland

Authors: KAMİL SEYREK

Abstract: The mammalian adrenal gland in the developmental stage provides excellent system for studying the role(s) of lectins in the foetal period because the adrenal gland is composed of two separate endocrine organs which differ in embryologic origin, type of secretion, and function. There is no information on the presence of galectin-1 or galectin-1 reactive glycoconjugates in the development of the bovine adrenal gland. Therefore, the present study focused on this protein. For separation of galectin-1 dialysis, affinity chromatography and SDS-PAGE were used. To monitor the localisation of galectin-1 and its epitopes immunhistochemistry was used. A single 14-kD band comigrated with a standard marker (a-lactoglobulin) of the molecule was seen on polyacrylamide gel. It was also found that galectin-1 exists abundantly in the smooth muscle cells of blood-vessels and in the migrating glia cells. Likewise, biotin-marked galectin-1 exhibits intensive staining in zona fasiculata cells.

Keywords: Galectin-1, isolation, localisation, bovine, adrenal gland

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