Comparison of IFAT and ELISA Results in Mice Experimentally Infected with Toxocara canis

Authors: HASAN AYÇİÇEK, MEHMET TANYÜKSEL

Abstract: Toxocariasis is a disease which indicates a wide symptomatic spectrum and occurs usually by the ingestion of infective Toxocara canis (rarely Toxocara cati) eggs by humans as a paratenic host. In this study, the sera samples of experimentally infected mice were evaluated on different days by IFAT and ELISA. Regarding the sera samples obtained on the 15^{th} and following days of the study, the groups seropositivity in IFAT by adult and larval sectional antigens and in TES-ELISA were 70, 73.3 and 96.7%, respectively. In the control group, seronegativity in IFAT by adult and larval sectional antigens, and in T. canis Excretuar/Secretuar-ELISA, was 81.4, 85.1 and 98.1%, respectively. When comparing the detection of seronegativity in the control group and seropositivity in the study group, ELISA was found to be more reliable than IFAT and there was a statistically significant difference between these two tests (P < 0.05).

Keywords: Toxocara canis, IFAT, ELISA, mice

Full Text: PDF