Susceptibility of Prototheca zopfii strains isolated from cows with mastitis to chlorhexidine and iodine

Authors: HENRYK KRUKOWSKI, ANDRZEJ LISOWSKI, BOZENA NOWAKOWICZ-DEBEK, LUKASZ WLAZLO

Abstract: Bovine mastitis caused by Prototheca spp. can assume high significance because of economic losses and the potential risk to public health. The aim of our study was to evaluate the effect of chlorhexidine and iodine on Prototheca zopfii strains isolated from bovine milk. For this study, 15 Prototheca zopfii isolates previously obtained from cows with clinical and subclinical mastitis in Poland were used. The in vitro susceptibility tests to chlorhexidine and iodine of the strains of Prototheca zopfii for the determination of their minimal microbicidal concentrations (MMCs) were performed using the tube dilution method in Sabouraud dextrose broth and the evaluation of colony growth after plating in Sabouraud dextrose agar. In this study, the MMC was 0.3125%-1.25% for iodine and 0.0048%-0.0195% for chlorhexidine.

Keywords: Prototheca zopfii, mastitis, MMC, chlorhexidine, iodine

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