Coagulase Gene Polymorphism of Staphylococcus aureus Isolated from Subclinical Bovine Mastitis

Authors: ÖZKAN ASLANTAŞ, CEMİL DEMİR, HÜLYA TÜRÜTOĞLU, ZAFER CANTEKİN, YAŞAR ERGÜN, GÖKHAN DOĞRUER

Abstract: This study was conducted to investigate the coagulase gene polymorphism of Staphylococcus aureus isolated from subclinical bovine mastitic milk samples from 3 different Turkish cities (Hatay, Gaziantep, and Burdur). Amplification of the coagulase gene from 80 S. aureus isolates produced 5 different polymerase chain reaction (PCR) products; 79 isolates showed only 1 amplicon, and 1 isolate showed 2 amplicons. The isolates were grouped into 9 genotypes by analysing the restriction fragment length polymorphism (RFLP) of the gene; the 2 most common genotypes accounted for 73.8% of the isolates. The results indicated that many coagulase gene genotypes are present in the studied regions and only 1 or 2 genotypes predominated.

Keywords: Staphylococcus aureus, RFLP-PCR, mastitis

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