Isolation and Serotyping of Ornithobacterium rhinotracheale from Poultry

Authors: SÜHEYLA TÜRKYILMAZ

Abstract: The aims of this study were the isolation of Ornithobacterium rhinotracheale from poultry and serotyping of the isolates and field sera by agar gel precipitation (AGP) test. For the isolation 257 chickens and 214 turkeys were used and for the AGP test 333 chickens and 250 turkeys were used. O. rhinotracheale was isolated from the sinus discharge of 3 broiler breeders at 37, 42 and 46 weeks of age. It was determined that 3 isolates were O. rhinotracheale serotype I having cross reactions with serotype L. Of 333 sera examined 86 were found seropositive in chickens by AGP test. It was determined that 30 sera were serotype A, 25 sera were serotype B, 9 sera were serotype I, and 6 sera were serotype L, while 13 sera were serotype A with cross reactions with serotype B, and 3 sera were serotype I with cross reactions with serotype L. Of the 250 turkey sera 64 were seropositive and of those 17 were serotype A, 29 were serotype B, 5 were serotype I, and 2 were serotype L, while 11 sera were serotype A with cross reactions with serotype B.

Keywords: Ornithobacterium rhinotracheale, poultry, isolation, agar gel precipitation

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