The apoptotic and proliferative effects of tulathromycin and gamithromycin on bovine tracheal epithelial cell culture

Authors: YAŞAR ŞAHİN, EBRU YILDIRIM, BEGÜM YURDAKÖK DİKMEN, MUSTAFA TÜRK

Abstract: Gamithromycin and tulathromycin are commonly used in the treatment of bovine respiratory bacterial diseases. The current work was undertaken to establish the apoptotic, necrotic, and cytotoxic effects of these antibiotics in the target animal. Cells with apoptosis and necrosis were determined by dual staining method, cytotoxic effects were determined by MTT assay, cell proliferative effects were examined by XCelligence real-time cell analysis system (RTCA-SP). The comparison between gamithromycin and tulathromycin concentrations on tracheal cells in terms of % cell viability was found to be significantly different. While the cell viability percentage of gamithromycin was higher at 150 μg/mL, 180 μg/mL, and 240 μg/mL than tulathromycin, and at 2 μg/mL, 4 μg/mL, 10 μg/mL, 20 μg/mL, and 50 μg/mL concentrations tulathromycin cell viability was higher than gamithromycin (p < 0.05). When the staining method data were evaluated, the difference between the results of % apoptotic index at 20 μg/mL concentration was significant and it was found that gamithromycin had more apoptotic effect than tulathromycin (p < 0.05). It was seen that tulathromycin and gamithromycin applied on tracheal epithelial cells at concentrations of 2 and 10 ?g/mL increased the viability depending on time. The increase in epithelial cell proliferation of gamithromycin and tulathromycin due to time shows that these antibiotics can maintain longterm prophylactic treatment against diseases.

Keywords: Bovine trachea, epithelial cell, gamithromycin, proliferation, tulathromycin

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