Molecular characterization of the HSPA1A gene by single-strand conformation polymorphism and sequence analysis in Holstein-Friesian crossbred and Deoni cattle raised in India

Authors: RAMESHA P. KEREKOPPA, AKHILA RAO, MARAPPA BASAVARAJU, GUJJAR RAMACHANDRA GEETHA, LAKHSMINARAYAN KRISHNAMURTHY, THOTA VL NARASIMHA RAO, DAYAL NITAI DAS, Kataktalware Mukund

Abstract: A group of proteins called heat shock proteins (HSPs) are synthesized during heat stress. They are expressed at high levels when exposed to stress. Polymorphism in HSPs has been reported to be associated with heat tolerance and reproductive performance in cattle. The present study aimed to investigate the polymorphisms in the coding region of the HSPA1A gene in Holstein-Friesian (HF) crossbreds and the Deoni breed of cattle. Genomic DNA was extracted from 94 animal blood samples and was subjected to polymerase chain reaction-single strand conformation polymorphism analysis, which revealed 14 band patterns in Deoni cattle and 8 band patterns in HF crossbreds. Sequence data were analyzed using BioEdit software for detecting single nucleotide polymorphisms. Sequence analysis showed 12 single nucleotide polymorphisms in the coding region of the HSPA1A gene, which included 5 transitions (G456A, A972G, A1098G, C1766T, and G1788A) and 2 transversions (C312G and G2033C) in Deoni cattle and 2 indels (C at positions 574?575 and 624?625), 2 transitions (A480G and A1098G), and 1 transversion (C312G) in HF crossbred cattle. The study indicated a high degree of genetic variability in the HSPA1A gene in the cattle populations under study.

Keywords: HSPA1A gene, Deoni cattle, Holstein-Friesian crossbreds, genetic variability, single-strand conformation polymorphism analysis

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