The protective effects of cysteamine, putrescine, and the combination of cysteamine and putrescine on fibrosarcoma induced in mice with 3-methylcholanthrene

Authors: ABDULLAH DOĞAN, PINAR AKSU, DİNÇER ERDAĞ, ALİ NAZMİ CAN DOĞAN, KADİR ÖZCAN, ERTAN DOĞAN

Abstract: In this study, the protective effects of cysteamine, putrescine, and the combination of cysteamine and putrescine were investigated in mice with fibrosarcoma induced with 3-methylcholanthrene (3-MC). A total of 135 adult male albino mice (Mus musculus), 2-3 months old and weighing 20 ± 2.0 g, were used in this study. All groups (each consisting of 15 mice) were fed ad libitum. The experimental groups were Group 1 (drinking water), Group 2 (0.2 mL sesame oil s.c.), Group 3 (1 mg 3-MC/0.2 mL sesame oil), Group 4 (0.1% cysteamine), Group 5 (0.1% putrescine), Group 6 (0.1% cysteamine + putrescine), Group 7 (1 mg 3-MC/0.2 sesame oil + 0.1% cysteamine), Group 8 (1 mg 3-MC/0.2 mL sesame oil + 0.1% putrescine), and Group 9 (1 mg 3-MC/0.2 mL sesame oil + 0.1% cysteamine + 0.1% putrescine). Experimental groups were given cysteamine and/or putrescine mixed with drinking water right after being injected with 3-MC subcutaneously. After 7 months, the mice were euthanized by means of cervical dislocation and were evaluated morphologically and histopathologically. The results showed that the number of tumors of 3-MC-induced fibrosarcoma were reduced from the highest to the lowest by cysteamine, cysteamine + putrescine, and putrescine, respectively.

Keywords: Fibrosarcoma, 3-methylcholanthrene, cysteamine, putrescine

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