Hemopoiesis in the pronephros of tench, Tinca tinca, Linnaeus 1758 (Teleostei, Cyprinidae): cytochemical identification and cell morphology

Authors: ANDI ALIJAGIC, DAMIR SULJEVIC

Abstract: Hemopoiesis in the tench (Tinca tinca: Teleostei, Cyprinidae), a cyprinid fish that can be found within the entire territory of Eurasia, from France in the west to the Yenisei River in the east, has not yet been described. The aim of this research was analysis of hemopoiesis lineages in the pronephros of Tinca tinca (sampled from the Jablanicko Reservoir). Identification and cells measurements were done by using a light microscope (Olympus BX41) and an Olympus DP12 camera. Hemopoietic cells of the tench are divided into five different series that include erythropoietic, granulopoietic, lymphopoietic, thrombopoietic, and monopoietic cell series. Erythropoietic series consist of a minimum of three stages: basophilic erythroblasts, acidophilic erythroblasts, and mature erythrocytes. Erythroblasts are nucleated and the most abundant hemopoietic cells in the pronephros of tench. Granulopoietic series consist of granuloblasts, namely two different types: neutrophilic granuloblasts (most frequent) and pseudoeosinophilic or heterophilic granuloblasts (POX-positive). Lymphopoietic series consist of lymphoblasts and also small, medium, and large lymphocytes. Thrombopoietic series consist of prothrombocytes and mature thrombocytes, the smallest hemopoietic cells of the tench. Monopoietic series consist of only one maturation stage called monocyte precursor cells, vacuolated and the largest hemopoietic cell in the pronephros of tench. It can be concluded that the pronephros represents a principal place of hemopoiesis in tench.

Keywords: Identification, hematopoietic stages, peroxidase activity, head kidney, fish hematology

Full Text: PDF