An investigation of antibacterial effects of steroids

Authors: ABDULLAH DOĞAN, SALİH OTLU, ÖZGÜR ÇELEBİ, PINAR AKSU, ALİYE GÜLMEZ SAĞLAM, ALİ NAZMİ CAN DOĞAN, NESLİHAN MUTLU

Abstract: In this study, the effects of steroid substances such as corticosterone, progesterone, estrone, beta-sitosterol, and stigmasterol on some bacteria were investigated. In the research, standard strains of the gram-positive bacteria Bacillus subtilis (lab isolate) and Staphylococcus aureus (ATCC 6538) and the gram-negative bacteria Escherichia coli (ATCC 25922), Pasteurella multocida (ATCC 12945), and Pseudomonas aeruginosa (ATCC 9027) were used. A modified broth dilution method was applied for determining the minimal inhibitory concentration (MIC) of the steroids. Mueller Hinton broth (Becton Dickinson, 211443) was used to prepare dilutions and as a medium. At the end of the study, the MIC of corticosterone for P. multocida and the MIC of beta-sitosterol for S. aureus were both determined as 32 μg/mL. However, other steroids showed no effects on the bacteria. These steroids did not appear to have widespread antibacterial properties.

Keywords: Corticosterone, progesterone, estrone, beta-sitosterol, stigmasterol, antibacterial

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