Application of Western Blotting for the Immunodiagnosis of Fasciola hepatica in Cattle Using Excretory/Secretory Antigens

Authors: H. OĞUZ SARIMEHMETOĞLU

Abstract: In this study, protein bands of excretory/secretory antigens of Fasciola hepatica were determined using SDS-PAGE and Western blotting. Blood and faecal samples were obtained from cattle brought to Kazan slaughterhouse. After examining the organs and faecal samples of these cattle for Fasciola hepatica and other helminths, serum samples were divided into two groups as positive (10 cattle) and negative (5 cattle) for F. hepatica. The sera of these two groups were tested using Western blotting. The bands obtained from the sera of positive and negative groups were compared and the specific protein bands for F. hepatica infection were 36, 29 and 17 kDa.

Keywords: Cattle, Fasciola hepatica, SDS-PAGE, Western blotting

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