Sex Determination by CHDW and CHDZ Genes of Avian Sex Chromosomes in Nymphicus hollandicus

Authors: HARUN CERİT, KOZET AVANUS

Abstract: The aim of this study was sex determination in Nymphicus hollandicus without giving it any harm and obtaining accurate results by DNA analysis. CHD genes are preserved within avian Z and W sex chromosomes. The intron regions of the CHDW and CHDZ genes vary between male (ZZ) and female (ZW) individuals. The method used in this study was based on this difference. DNA was extracted from feathers instead of blood. The intron regions of CHDW and CHDZ genes were amplified by sex specific primers (P2 and P8). PCR products were screened by agarose gel electrophoresis. Individuals showing double (ZW) and single (ZZ) bands were identified as females and males, respectively.

Keywords: Nymphicus hollandicus, CHD1W, CHD1Z, avian, sex identification

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