In Vitro Esterase Activity of Carbonic Anhydrase on Total Esterase Activity Level in Serum

Authors: MUHAMMET FEVZİ POLAT, BARBAROS NALBANTOĞLU

Abstract: The in vitro esterase activity of carbonic anhydrase on total esterase activity level was investigated. This effect was obtained by subtracting the value of net esterase from the value of total esterase in sera with normal (95.22 ± 8.87) and high glucose levels (208.58 ± 87.90). Total esterase activity, net esterase activity and esterase activity of carbonic anhydrase were 20.2 ± 1.9, 18.1 ± 1.8 and 2.1 ± 0.7 U/min/mL serum in sera with a normal glucose level, and 21.3 ± 2.8, 17.9 ± 2.9 and 3.4 ± 1.2 U/min/mL serum in sera with a high glucose level, respectively. Thus, the esterase activity of carbonic anhydrase was determined to be 10.4% in sera with a normal glucose level, and to be 16% in sera with a high glucose level. The total esterase activity level of the sera with a high glucose level was statistically significant (p < 0.05) compared to those with a normal glucose level, whereas the difference between net esterase activity levels was not significant. As seen, without taking into consideration the effect of the in vitro esterase activity of carbonic anhydrase, the total esterase levels would have been found significant under pathological conditions and this may lead us to interpret the in vivo results in error.

Keywords: Esterase activity, esterase, and carbonic anhydrase

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