Importance of 14-3-3eta, anti-CarP, and anti-Sa in the diagnosis of seronegative rheumatoid arthritis

Authors: EMRAH SALMAN, SALİH ÇETİNER, BARIŞ BORAL, FİLİZ KİBAR, EREN ERKEN, EMİNE DUYGU ERSÖZLÜ, SUADE ÖZLEM BADAK, REYHAN BİLİCİ SALMAN, YAŞAR SERTDEMİR, ALEV ÇETİN DURAN, AKGÜN YAMAN

Abstract: Background/aim: Rheumatoid arthritis (RA) is an autoimmune disease characterized by synovial inflammation. The study aimed to assess serum 14-3-3eta, anti-CarP, and anti-Sa in seronegative RA (SNRA) patients who were treatment-naïve as well as in healthy subjects. This is the first study in the literature to examine these autoantibodies together in SNRA patients. Materials and methods: Forty-five treatment-naïve SNRA patients and 45 healthy subjects were recruited. Drugs change the levels of autoantibodies; therefore, patients who took any medication had been excluded from our study. Anti-carbamylated protein, anti-Sa, and 14-3-3eta were measured by using three different ELISA kits.Results: Median serum concentration of healthy controls in 14-3-3eta was 0.02 (0.02?0.27) ng/mL. Median serum concentration of SNRA patients in 14-3-3eta was 1.00 (0.48?1.28) ng/mL. Data were analyzed with Mann?Whitney U tests; the P-value was <0.001 in 14-3-3eta. Receiver operating characteristic (ROC) curve analysis showed that 14-3-3eta in SNR compared to healthy controls had a significant (P < 0.001) area under the curve (AUC) of 0.90 (95% confidence interval, 0.83?0.96). At a cutoff of ?0.33 ng/mL, the ROC curve yielded a sensitivity of 88.9%, a specificity of 82.2%, a positive predictive value of 83.3%, and a negative predictive value of 88.1%.Conclusion: We found that 14-3-3eta can be used as a diagnostic marker in SNRA.

Keywords: 14-3-3eta, Anti-carP, Anti Sa, seronegative

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