The Change of Glutathione Dependent Anti-Oxidant Mechanism in Patients with Chronic Renal Disease by Hemodialysis

Authors: Hatice PAŞAOĞLU, Sabahattin MUHTAROĞLU, Mehmet GÜNEŞ, Cengiz UTAŞ

Abstract: In order to test the for existence of a possible effect during hemodialysis we investigated glutathione and related enzymes before and after dialysis of 36 patients (20 M/16 F) with chronic renal failure (CRF). 25 healthy person (15 M/10F) served as controls. Erythrocyte reduced glutathione (GSH) concentrations, glucose -6- phosphate dehydrogenase (G-6-PD), glutathione peroxidase (GPX) activities and plasma GPX activities were significantly decreased in patients (p<0.01). These levels were significantly increased with dialysis (p<0.01) but were still lower than in controls. Predialysis plasma GSH levels of patients were not significantlydifferent from the control group. But postdialysis plasma GSH levels increased from predialysis levels (p<0.01). Plasma Selenyum (Se) concentrations were lower in patients than in controls (p<0.01) and not significantly changed with dialysis. These results suggest that the antioxidant system related to GSH is insufficient in CRF patients and this system improves only partly after hemodialysis.

Keywords: Glutathione, Glutathione peroxidase, Glucose 6-Phosphate dehydrogenase, Hemodialysis

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