Frequency of twelve carcinogenic human papilloma virus types among women from the South Backa region, Vojvodina, Serbia

Authors: GORDANA KOVACEVIC, NATASA NIKOLIC, ALEKSANDRA JOVANOVIC-GALOVIC, IVANA HRNJAKOVIC-CVJETKOVIC, DUSAN VULETA, ALEKSANDRA PATIC, JELENA RADOVANOV, VESNA MILOSEVIC

Abstract: Background/aim: The aim of this study was to determine the presence and age distribution of different oncogenic human papilloma virus (HPV) types in women in the South Backa region and its relationship to Pap results. Materials and methods: In a group of 1087 women with normal and abnormal cytology, the commercial HR HPV Real-TM kit (Sacace Biotechnologies, Italy) was used. Results: Overall, 50.5% of the women were HPV positive. The presence of HPV types 18, 31, 51, and 58 was significantly influenced by age, while the presence of HPV types 16 and 45 was significantly influenced by cervical cytology. Results of the LSD test show a wide spectrum of high risk HPV among women with normal cytology and women with a low grade cervical lesion rate (atypical squamous cell of undetermined significance (ASCUS) and low grade squamous intraepithelial lesions (LSIL). The most prevalent HPV types found were 16, 31, 51, 18, and 52. In the HSIL group the most prevalent HPV types were 16 and 45. Conclusion: The reported results provide new data on the circulation of oncogenic HPV genotypes and frequency of multiple infections among women in Vojvodina and suggest that a prophylactic vaccine against HPV 16 and 18 has the potential to prevent approximately half of the high-grade lesions.

Keywords: Human papilloma virus, real-time PCR, cervical cytology

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