Immunophenotyping in Acute Leukemias

Authors: Türkan PATIROĞLU, Ali ÜNAL, Mehmet Akif ÖZDEMİR

Abstract: The incidence and clinical implication of unusual patterns of expression of leukocyte differentiation antigens in acute leukemia were assessed on 54 newly diagnosis pediatric and adult cases undergoing Immunophenotyping with a panel of monoclonal antibodies. The diagnosis was based on FAB morphology and cytohemistry by the pediatric and adult hematologist. 0f the 37 cases in childhood, 27 (70.3 %) had acute lymphoblastic leukemia (ALL) and of the 17 adults, 5 (29.4 %) had ALL. 0ne patients in childhood (3.7 %) and an adult (20 %) with ALL expressed myeloid surface antigens. T-lineage markers as well as CD10 antigen. Ten cases (27 %) in childhood showed expression of CD13 and CD 33. Furthermore, 4 of them (40 %) expressed CD14 antigen in addition to CD13 and CD 33 antigens. Twelve adults (70 %) expressed either CD13 or CD33 antigens and 4 cases (33.3 %) with acute myelomonocytic leukemia (AMML) showed expression of CD14 antigen. T-lineage markers were expressed in a child (10 %) and in 3 adults (25 %) with acute myeloblastic leukemia (AML).

Keywords: Immunophenotyping, Acute leukemia