Radioimmunotherapy of mice bearing breast tumors with ^{177}Lu-labeled trastuzumab

Authors: SAMIRA RASANEH, HOSSEIN RAJABI, SHAHRAM AKHLAGHPOOR, SHAHAB SHEYBANI

Abstract: In the present study, we investigated the therapeutic efficacy of trastuzumab labeled with lutetium-177 (^{177}Lu) in mice bearing breast tumors. Materials and methods: Trastuzumab was labeled with ^{177}Lu via DOTA as a chelator. In vitro and in vivo quality control tests of incorporated activity, immunoreactivity, stability of ^{177}Lu-trastuzumab, biodistribution, and imaging studies were performed. Therapeutic efficacy and dosimetry studies were carried out in mice with breast tumors. Results: The good in vitro and in vivo quality control results suggested that ^{177}Lu-trastuzumab could be used as a radioimmunotherapy agent. Reductions of 81% and 98% in the mean tumor volume for the group that received ^{177}Lu-trastuzumab [7.4 MBq] were observed at 42 and 45 days after treatment, respectively. Conclusion: The results showed that ^{177}Lu-trastuzumab could be considered as a possible new radiopharmaceutical agent for use in radioimmunotherapy of breast cancer in humans.

Keywords: Radioimmunotherapy, breast cancer, lutetium-177, trastuzumab, gamma camera imaging

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