Role of mesenchymal stem cell-derived soluble factors and folic acid in wound healing

Authors: AHU PAKDEMİRLİ, FERİHA TOKSÖZ, ASLIHAN KARADAĞ, HÜSEYİN KORAY MISIRLIOĞLU, YASEMİN BAŞBINAR, HÜLYA ELLİDOKUZ, OSMAN AÇIKGÖZ

Abstract: Background/aim: Mesenchymal stem cells (MSCs) are a type of adult stem cell consisting of a heterogeneous subset of stromal stem cells that can be isolated from adult tissues. Folic acid is another important contributor to tissue regeneration and repair, which affects the synthesis of some building block molecules used for wound healing. In this study, we examine the effect of folic acid and MSC-derived soluble factors in the wound healing model.Materials and methods: Human umbilical vein endothelial cells (HUVECs) and bone marrow-derived MSCs (BMSCs) were cultured for this study. Cell proliferation analysis was done with xCELLigence RTCA. After 48 h of cultivation, the cell culture medium was collected as MSC conditional medium containing mesenchymal stem cell-derived soluble factors (MDFs). Different concentrations of MDFs (12%, 25%, 50%, 75%, and 100%) were applied to the HUVEC cell line. Folic acid (25, 30, 50, 60, 75, 90, and 100 μM) was tested by application of three different groups (control, 25 μM folic acid, 625 μM folic acid inhibitors) for proliferation on the HUVEC cell line. The combined effects of folic acid and MDFs were tested on the HUVEC cell line with 25 μM folic acid and 50 μM MDFs. All data were statistically analyzed using SPSS 15.0 for Windows.Results: Significant differences were observed between controls and cells treated with folic acid, as well as between controls and both folic acid and MDFs (P < 0.05). Among the treated groups, the fastest wound closure rate was seen in cells treated with both folic acid and MDFs.Conclusion: The results show that both folic acid and MDFs increased the wound healing rate in HUVECs when they were used sepa-rately. The strongest benefits were seen in treatment using folic acid and MDFs together.

Keywords: Mesenchymal stem cell, mesenchymal stem cell-derived soluble factor, wound healing, folic acid, cell differentiation

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