Fecal carriage of extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella spp. in a Turkish community

Authors: DERYA ÇAKIR ERDOĞAN, FÜSUN CÖMERT, ELİF AKTAŞ SEPETCİ, FÜRÜZAN KÖKTÜRK, CANAN KÜLAH

Abstract: Background/aim: The aim of this study was to determine the prevalence of fecal carriage of extended-spectrum beta-lactamase (ESBL)-producing bacteria, enzyme types, and risk factors affecting colonization. Materials and methods: A total of 576 stool samples from outpatients were examined between October 2012 and May 2013. Screening was done with selective EMB plates. ESBL were detected by double-disk synergy and confirmed agar strip gradient methods. Enzyme types were determined by PCR. Results: The prevalence of fecal carriage was found as 30% (173 of 576). Recent use of antibiotics, hospitalization and surgical operation, diabetes, crowded household populations, and old age were associated with higher carriage rates. Of the ESBL-producing bacteria, 87.5% were positive for bla_CTX-M genes. Of the bla_CTX-M gene-positive isolates, 95.2% were positive for bla_CTX-M-1 genes; among these, 82.2% were positive for bla_CTX-M-3 and 67.7% were positive for bla_CTX-M-15 genes while 62.5% isolates were positive for both bla_CTX-M-3 and bla_CTX-M-15 genes Conclusions: A high rate (30%) of fecal carriage of ESBL bacteria was found in an adult population. The predominant beta-lactamase enzyme types were CTX-M-3 and CTX-M-15.

Keywords: Extended-spectrum beta-lactamase, Escherichia coli, Klebsiella spp., fecal carriage

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