The Effect of FAD on Erythrocyte Glutathione Reductase Activity in Undialyzed Uremic Patients and Healthy Subjects

Authors: Zeki ARI, Bekir Sami UYANIK, M. Ramazan YİĞİTOĞLU, Necip KUTLU, İbrahim PİRİM

Abstract: The eryrocyte glutathione reductase (GSSG-R) activity with and without FAD supplementation (Act. A and Act B, respectively) was studied in 24 undialyzed uremic patients and 25 healty subjects. Mean erytrocyte GSSG-R activity from normal subjects was 5039+-1.43 U/g Hb (the active form). After the addition of FAD to the hemolysates, this increased to 7.79+-1.07 U/g Hb, an increase of 1.88+-0.84 U/g Hb (the inactive from) (p<0.001). In patients with FAD supplementation, erytrocyte GSSG-R activity increased from 9.26+-1.19 U/g Hb to 10.06+-1.06 U/g Hb, an increased of0.80+-0.76 U/g Hb (p<0.01). In addition, there were significant positive correlations between the enzyme activities and plasma urea level, and negative correlations between the enzyme activities and hemoglobin concentrations in patients, but not in the controls. It was included that further cross-sectional in vivo studies should be made to determine whether riboflavin administration may or nat be useful in preventing renal anemia.

Keywords: Anemia, FAD, Glutathione Reductase, Riboflavin, Uremia