Detection of Serum HCV RNA by Polymerase Chain Reaction in Patients with Chronic Hepatitis C

Authors: S. Ayşe ÖZER, Cem KALAYCI, Melike AVŞAR, Müge CANER, Sertaç Kip ÜLGEN, Erol AVŞAR, Şafak KIZILTAŞ, Beyazıt ÇIRAKOĞLU

Abstract: Hepatitis C virus (HCV) RNA was isolated from the sera of 15 anti-HCV positive patients. cDNAs were synthesized by reverse transcription and amplified by two round nested polymerase chain reaction (PCR). HCV infection was confirmed by the detection of 256 base pairs long DNA fragments on the 2% agarose gels in the electrophoresis of PCR products with the positive and negative controls. 10 of 15 infection were confirmed by this molecular technique.

Keywords: HCV RNA, Nested PCR, Chronic Hepatitis C.