Quantitative Analysis of l-Hyoscyamine in Hyoscyamus reticulatus L. by GC-MS

Authors: MURAT KARTAL, SEMRA KURUCU, LEVENT ALTUN, TİMURHAN CEYHAN, ESİN SAYAR, ŞEMSETTİN CEVHEROĞLU, YALCIN YETKİN

Abstract: The hyoscyamine content of leaves and root samples of Hyoscyamus reticulatus} L. from Bulanık, Muş in east Anatolia, Turkey, was determined by capillary GC-MS to be 0.036 \pm 0.004% in the leaves and 0.056 \pm 0.011\% in the roots as the predominant compound. Sopolamine was only detected in trace amounts. These findings are in accordance with reports on hyoscyamine content in Hyoscyamus} species. The limit of detection was 3.125 \mu g/mL and the limit of quantification was 6.25 \mu g/mL for hyoscyamine. This method has been shown to be linear and sensitive.

Keywords: l-Hyoscyamine, Hyoscyamus reticulatus L., Capillary GC-MS

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