Voltammetry of the Anticancer Drug Mitoxantrone and DNA

Authors: ARZUM ERDEM, MEHMET ÖZSÖZ

Abstract: The interaction of mitoxantrone (MTX) with calf thymus double-stranded DNA (dsDNA) and calf thymus single-stranded DNA (ssDNA) was studied electrochemically by using differential pulse voltammetry (DPV) and cyclic voltammetry (CV) at a carbon paste electrode (CPE). The changes in the experimental parameters (the concentration of MTX, the concentration of DNA and the accumulation time of MTX) were studied by DPV; in addition, the detection limit and the reproducibility were determined. It was observed for the interaction of MTX with dsDNA and ssDNA that the signal of the bare electrode was higher than the signal of the dsDNA modified CPE. The signals in the cyclic voltammograms were found to be decreasing in the following order: bare CPE, ssDNA modified and dsDNA modified CPE.

Keywords: Mitoxantrone, Biosensors, DNA, Voltammetry, Intercalation

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