The Effect of Cultural Conditions on the Variations of SOD, CAT and GSH-Px Activities and LPO Levels in the Filamentous Fungus Fusarium equiseti

Authors: HÜLYA AYAR KAYALI, LEMAN TARHAN

Abstract: The changes in the activity of cellular detoxifying defence enzymes such as SOD, CAT and GSH-Px against superoxide anion radical and hydrogen peroxide, and in LPO levels in the filamentous fungus, Fusarium equiseti were measured under growth condition. Experiments were performed comparing changes in glycerol and saccharose (5-25 g/L) as carbon sources, and in glycine, peptone (5-35 g/L) as nitrogen sources in an AFM medium. While SOD activities correlate well with respect to the increase in the glycerol concentration (r = 0.437, p < 0.001), CAT activities showed negative correlation (r = -0.663, p < 0.001). The increase in SOD and CAT activities of F. equiseti correlated with increases in saccharose concentration. When glycerol and saccharose were used as carbon sources, the maximum SOD, CAT and GSH-Px enzymes activities and the minimum LPO level were determined in the medium containing 15 g/L of saccharose on the 12^{th} day at 66.61, 182.79, 1.045 IU/mg and 1.41 nmol MDA/ gr wet weight, respectively. The effects of peptone and glycine as nitrogen sources were also investigated: 53.4% and 48.03% decreases were observed in the SOD and CAT activities of F. equiseti in the presence of 10 g/L of peptone in the culture medium. The presence of 15 g/L of glycine caused 5.30% and 69.90% decreases in the SOD and CAT activities in comparison to the presence of 15 g/L of saccharose, respectively. On the other hand, LPO levels increased in proportion to the decrease in antioxidant enzyme activities in glycine and peptone supplemented media.

Keywords: Fusarium, glycerol, saccharose, glycine, peptone, SOD, CAT, GSH-Px, LPO (MDA)

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