An Electrochemical DNA Biosensor for the Detection of DNA Damage Caused by Radioactive Iodine and Technetium

Authors: PINAR KARA, KAĞAN DAĞDEVİREN, MEHMET ÖZSÖZ

Abstract: A DNA damage process by radioactive iodine (I-131) and technetium (Tc-99m) is described. The procedure relies on a label-free electrochemical DNA biosensor. Fish sperm double stranded DNA was immobilized onto a carbon paste electrode (CPE) by electro deposition and the changes to the guanine oxidation signal were observed upon exposure to radioactive I-131 and Tc-99m by differential pulse voltammetry (DPV). The changes to the signal of guanine after exposure to therapeutic doses of Tc-99m and I-131 showed conformational changes in DNA molecules.

Keywords: Electrochemistry, DNA damage, Tc-99m, iodine-131

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