Isolation of Trichomes from Wheat and Other Species of Flowering Plants

Authors: ZAURE G. AYTASHEVA, ELIZAVETA D. BOGDANOVA, AITKALI M. KALIMAGAMBETOV, SERGEY V. CHEKALIN, FATIMA A. POLIMBETOVA

Abstract: Plant hairiness or pubescence as a specific phenotypical feature related to dehydration tolerance and resistance to leaf vermin is considered in this paper. A method for the isolation of trichomes, earlier developed for Arabidopsis Heynh., was found to be appropriate for the separation of similar polarised cells from pubescent wheat, Triticum aestivum L., lines, as well as some other higher plant species. This procedure thus paves the way for the study of the molecular organisation of trichomes in wheat and other mono- and dicotyledoneous plants.

Keywords: Trichomes, leaf, wheat, Triticum aestivum L., resistance, vermin, Oulema melanopiis L.

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