A Study of Selected Isozymes in Capsicum baccatum, Capsicum eximium, Capsicum cardenasii and Two Interspecific F_1 Hybrids in Capsicum Species

Authors: A. NACİ ONUŞ, BARBARA PICKERSGILL

Abstract: Selected isozymes were investigated in plants of Capsicum baccatum L. ( Solanaceae) accessions SA219 (P.G.Smith), Hawkes 6489 (P.G.Smith), Capsicum cardenasii Heiser and Smith accession SA268 (P.G.Smith), Capsicum eximium A.T.Hunz accession Hawkes 3860 (J.G.Hawkes) and two interspecific F1 hybrids, C. baccatum SA219 x C. eximium Hawkes 3860 and C. baccatum Hawkes 6489 x C. cardenasii SA 268. The standard technique of horizontal gel electrophoresis was employed. The gel was cut into several slices and stained for different enzyme systems (aconitase, alanin aminopeptidase, esterase, glutamic oxaloacetic transaminase, glycerate-2-dehydrogenases, isocitrate dehydrogenase, malate dehydrogenase, peroxidase, phosphoglucomutase, phosphoglucose isomerases and shikimate dehydrogenases). After fixing the gel slices in 50% aqueous glycerol, the number and position of stained bands were recorded.

Keywords: Capsicum baccatum, Capsicum eximium, Capsicum cardenasii, isozymes, electrophoresis.

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