Observation of the Antimutagenic Potencies of Plant Extracts Against Pesticides in the Salmonella typhimurium strains TA98 and TA100.

Authors: Ali KALAYCIOĞLU, Cihan ÖNER, Günhan ERDEM

Abstract: Spinacia oleracea, L. (Chenopodiaceae), Lepidium sativum L. (Brassicaceae) and Urtica dioica L. (Urticaceae) were tested for their antimutagenic effects on pesticides in the Salmonella typhimurium strains TA98 and TA100 with and without the metabolic activation of S9. It was determined that the extracts from these plants decreased the mutagenic effects of Captan, Folpet, DDVP, Azinphosmethyl, Bioresmethrin and Trifluralin. Since various papers have previously discussed the antimutagenic effects of glutathione, cysteine and ascorbic acid, the extracts were also tested for the presence of these chemicals. Glutathione and cysteine were detected using paper chromatography. The presence of ascorbic acid was detected with a colorimetric assay. Mutagenic and antimutagenic assays of the chemical substances were evaluated using the Ames test.

Keywords: Ames test, pesticide, mutagenicity, antimutagenicity, plant extract.