Micropropagation of an antidiabetic medicinal plant, Artemisia pallens

Authors: VARSHA NITIN NATHAR, GHULAM MOHIUDDIN YATOO

Abstract: Artemisia pallens Wall. is an important aromatic medicinal plant used as a folk remedy for the treatment of diabetes mellitus. The present study was initiated to explore in vitro propagation of Artemisia pallens using different explants on Murashige and Skoog (MS) media supplemented with varying concentrations and combinations of growth regulators. Highest callogenic response (100%) was shown by shoot tip explants with 2 mg/L 2,4-dichlorophenoxyacetic acid. The highest number of shoots (14.25 ± 1.65) and highest shoot length (4.25 ± 0.47 cm) were observed on MS medium with 3 mg/L kinetin. After 40 days, shoots grown in vitro were transferred to rooting media. The highest number of roots (12 ± 0.08) and root length (8.15 ± 1.13 cm) were recorded on MS medium with 3 mg/L indole-3-butyric acid. The regenerated plantlets after 30 days were hardened in plastic cups containing sterile garden soil, farmyard soil, and sand (2:1:1) and were transferred to a greenhouse.

Keywords: Acclimatization, Artemisia, callogenesis, MS medium, regeneration

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