Foliar spray of phyto-extracts supplemented with silicon: an efficacious strategy to alleviate the salinity-induced deleterious effects in pea (Pisum sativum L.)

Authors: MUHAMMAD ADNAN SHAHID, RASHAD MUKHTAR BALAL, MUHAMMAD ASLAM PERVEZ, TAHIRA ABBAS, MUHAMMAD ANJUM AQUEEL, MUHAMMAD MANSOOR JAVAID, FRANCISCO GARCIA-SANCHEZ

Abstract: A pot culture study was conducted to assess the ameliorative effect of silicon, Melia azadirachta leaf extract, and sugar beet root extract, each applied individually or in different combinations, on salinity-induced detrimental effects in pea (Pisum sativum L.). Salinity markedly inhibited the growth, various gas exchange attributes, total phenol contents, membrane stability index, and productivity. On the other hand, lipid peroxidation, electrolyte leakage, H₂O₂ content, antioxidant activities, and leaf free proline and glycinebetaine contents were significantly enhanced by salinity. However, exogenously applied Si and phyto-extracts markedly alleviated the salinity-induced drastic effects on growth, gas exchange attributes, and productivity. Both phyto-extracts supplemented with silicon gave highly salinity mitigating effects by markedly improving growth, gas exchange attributes, enzymatic activities, osmolytes, and yield. The phyto-extracts and Si suppressed lipid peroxidation, electrolyte leakage, and H₂O₂ content by strengthening the enzymatic and nonenzymatic (proline and glycinebetaine) antioxidant defense system. The phyto-extracts and Si application also checked the root/leaf sodium and chloride contents, but improved the silicon contents. Thus, it can be concluded that exogenous application of silicon in combination with phyto-extracts of M. Azadirachta and sugar beet is a highly effective ameliorative approach to alleviate salinity-induced hazardous effects in plants, especially in pea, grown under a saline regime.

Keywords: Salinity, silicon, Melia azadirachta indica, sugar beet, gas exchange, antioxidants

Full Text: PDF