Regeneration and Agrobacterium-mediated Transformation Studies in Tomato ( Lycopersicon esculentum Miller)

Authors: HÜSEYİN AVNİ ÖKTEM, YETER BÜLBÜL, EMRE ÖKTEM, MERAL YÜCEL

Abstract: An optimized regeneration protocol and a suitable transformation technique is a necessity in obtaining transgenic plants in a given plant species. This work summarizes a simple culture method to obtain rooted plants from leaf pieces of two tomato cul-tivars (ES58 and WC156) within 3-4 months. Here, we also present our preliminary results on transformation experiments carried out with 8-day-old cotyledon explants and Agrobacterium tumefaciensEHA105 strain.

Keywords: Tomato, regeneration, Agrobacterium, transformation, Lycopersicon

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