Identification of benzoin obtained from calli of Styrax officinalis by HPLC

Authors: HATİCE DEMİRAY, AYLİN EŞİZ DEREBOYLU, ZEKİYE IŞIN YAZICI, FATİH KARABEY

Abstract: The stem tissue of Styrax officinalis L. distributed in West Anatolia was induced with agents such as boric acid and cocarboxylase (thiamine diphosphate), extensive stimulators of resin channels, in order to increase the amount of benzoin volatile oil. While the benzoin content was 120% in the induction medium to which excess boron and niacin were added, it increased to 231% when cocarboxylase (thiamine diphosphate) was added to the medium. Benzoin content of Styrax from petiole calli was 166%. HPLC-DAD results revealed that benzoin resin was present in 90% of the stems of Styrax officinalis distributed in West Anatolia. The major components of excess boron- and niacin-induced stem calli were hexane (58.33%), 3-methyl 2-pentene (16.10%), and cyclohexane (8.88%). Hexane (62%), methyl cyclopentane (19.09%), cyclohexane (12.04%), 2-hexanone (0.04%), ethylbenzene (0.03%), and benzene and 1-chloro-2-methylpropyl benzene (propene) were identified by the cocarboxylase application and GC-MS method. With enzyme application, while the percentages of decane and benzyl alcohol decreased, the cyclohexane ratio increased to 12.04%. Acetone (0.03%), ethyl acetate (4.10%), and dichloro methane (0.17%) contents were high as well.

Keywords: Styrax officinalis, stem calli, benzoin, cocarboxylase, boron

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