Identification of Lactoce Plasmids in Lactococcus lastic subsp. lactis Stains

Authors: ÇAĞLA TÜKEL, MUSTAFA AKÇELİK

Abstract: Plasmids encoding lactic acid fermentation ability were investigated in 50 strains of L. lactis subsp. lactis. Acriflavine was used for the elimination of lactose fermentation ability (Lac + ) from wild type strains. Lactose negative (Lac - ) mutants were selected on lactose indicator agar media. Six different lactose plasmids were identified for fifty strains by comparison of plasmid profiles of Lac - and Lac + mutants. The molecular sizes of these lactose plasmids ranged between 26.3 and 38.5 kb.

Keywords: Lactococcus lactis subsp. lactis, lactic acid fermentation, plasmid.

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