Determination of Salt Tolerance in Some Grapevine Cultivars ( Vitis vinifera L.) Under in vitro Conditions

Authors: NURAY SİVRİTEPE, ATİLLA ERİŞ

Abstract: Salinity tests were conducted on Çavus, Müsküle and Sultani Çekirdeksiz grapevine cultivars under in vitro conditions. Plant materials used in the salinity test were propagated using the axillary bud culture method. Single-node shoots were subjected to five different NaCl concentrations (0.00,0.25, 0.50, 0.75 and 1.00%) in MS+5 M BA medium for two periods (4 and 8 weeks). Proliferation, growth, total chlorophyll content and viability of explants decreased due to the increase in NaCl concentration and length of treatment period. Moreover, it was determined that salt treatment caused necroses in explants and the severity of this injury varied depending on the cultivar, NaCl concentration and the treatment period. The most tolerant grapevine cultivar to salt treatment was Çavus, and this was followed by Sultani Çekirdeksiz and Müsküle. It was determined that salt tolerant cultivars relatively maintained their growth rates and could avoid metabolic disordes such as chlorophyll deficiency.

Keywords: NaCl, selection, salt tolerance, in vitro, grapevine.

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