Genetic Identification of Diploid and Tetraploid Wheat Species with RAPD Markers

Authors: RAMİZ TAGİ ALİYEV, MEHRAJ ALİ ABBASOV, ALAMDAR CHARKAZ MAMMADOV

Abstract: The genetic diversity of 10 diploid and tetraploid wheat species was estimated using random amplified polymorphic DNA (RAPD) markers. Two species from diploid [Triticum boeticum (wild), Triticum monococcum] and five from tetraploid wheats [Triticum dicoccoides var. arabicum (wild), Triticum dicoccum var. farrum, Triticum dicoccum var. atratum, Triticum durum var. hordeiforme (Bereketli 95), Triticum durum var. leucurum (Sharq), Triticum turgidum var. alboyadurum, Triticum turgidum var. salomonis and Triticum persicum] were included for the analyses. Jacard's cluster analysis algorithm was used to determine genetic similarities. There were two main classes in dendrogram: the varieties [T. boeticum, T. dicoccoides var. arabicum, T. dicoccum var. farrum, T. dicoccum var. atratum, T .durum var. hordeiforme (Bereketli 95), T. durum var. leucurum (Sharq)] assembled in one group and the species [T. monococcum, T. turgidum var. alboyadurum, T. turgidum var. salomonis and T. persicum] in another. The same genotypes were also assessed in field conditions for structural analyses, which were carried out based on eight yield components. The dendrogram created was comparatively analyzed with the RAPD dendrogram. There were differences between genetic and phenotypic similarity of the studied accessions. Results indicated that some of genetically similar genotypes were different phenotypically.

Keywords: Plant genetic resources, biodiversity, wheat, RAPD, dendrogram, genetic relationships

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