Serological Properties of Pseudomonas syringaepv phaseolicolaIsolates Collected From Eskisehir

Authors: Dilek ERTUGRUL, Kıymet GÜVEN

Abstract: Thirty six bean seed samples collected from bean fields in Eskisehir-Türkiye were examined for detection and isolation of Pseudomonas syringae pv. phaseolicola. A polyclonal antiserum specific to P. s. pv. phaseolicola NCPPB 52 was raised in a rabbit and both biochemical and serological test were used for identification. Heat-stable O antigens of 92 isolates identified as P. S. pv. phaseolicola together with authentic isolates of the bacterium were compared by Ouchterlony immunodiffusion test and all the isolates had a common antigenic structure.

Keywords: Pseudomonas syringae pv. phaseolicola, halo blight, polyclonal antiserum, Ouchterlony immunodiffusion test

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