Transcription of the Autographa californica Nuclear Polyhedrosis Virus p39 Gene in Bombyx mori Cell Culture

Authors: Zihni DEMİRBAĞ

Abstract: Transcription of the Autographa californica nuclear polynedrosis virüs (AcNPV; Family: Baculoviridae) p39 gene (delayed-early gene) has been studied extensively in the productive system of Spodoptera frugiperda cells. This is the first transcriptional study of this gene in the Bombyx mori cell line. In this study, comparative transcription of the AcNPV p39 gene was examined in S. frugiperda and B. mori cell cultures. Northern blot hybridization of totoal RNA samples, isolated from mock-infected (O and 48 hour post infection, h.p.i.) and AcNPV-infected (0, 2, 6, 12, 24, 48 h.p.i.) S. frugiperda and B. mori cell lines, was analyzed. Results indicates that the 1.08 kb RNA fragment, specific to the p39 gene, is present in AcNPV-infected S. frugiperda and Bombyx mori cell lines. Moreover, the amount of transcript is higher and transcription occurs later in the cell line than in the S. frugiperda cell line.

Keywords: AcNPV, Baculovirus, p39 gene, transcription, Bombyx mori

Full Text: PDF