Phanerochaete chrysosporium and Funalia trogii for the Degradation of Cotton stalk and Their Laccase, Peroxidase, Ligninase and Cellulase Enzyme Aktivities Under Semi-Solid State Conditions

Authors: Sibel ŞIK, Ali ÜNYAYAR

Abstract: The potential of white rot fungi, namely Phanerochaete chrysosporium ME 446 and Funalia trogii for the degradation of lignocellulosics and their ligninolytic enzyme activities under semi-solid state conditions has been studied. In this study, cotton plant stalk which is an agrowaste, is used as lignocellulosics material. It's very good source of fibrous raw material for the manufacture of pulp and paper industry, and also is available abundantly. In this study, proportion of lignin and cellulose is in cotton stalk 23±2 % and 40±3 % respectively. Degradation of lignin and cellulose by P. chrysosporium and F. trogii under semi-solid state were determined during the 20 days of incubation period.Growth of P. chrysosporium on the cotton stalks resulted in the degradation of 22% lignin and 24% cellulose and also growth of F. trogii on the cotton stalks resulted in the degradation of 23% lignin and 27% cellulose. During the incubation period all extracellular enzyme activities, laccase, peroxidase, cellulase, ligninase, in the stock basal medium were determined in 48 hr. time intervals Morever, culture medium supplemented with glucose to investigate catabolic effects of glucose on the fungal metobolism, and also on the ligninolytic enzyme activities. For the degradation of polimeric molecules, such as lignin and cellulose, structurally simple molecules, like glucose is required. Because all enzyme activities that is described above related with dried mycelium weight decreased when compared with medium that is supplemented with glucose. From thes results, we conclode that ligninolytic enzyme systems of P. chrysosporium and F. trogii were activated during the secondary metobolism of fungal growth.

Keywords: Lignin degradation, laccase, peroxidase, ligninase, cellulase

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