cDNA cloning, molecular characterization, and expression analyses of two novel porcine ARRDC genes-ARRDC1 and ARRDC

Authors: HAILONG HUO, PEI WANG, SHUYAN WANG, YONGWANG MIAO, HONGJIANG WEI, YONGYUN ZHANG, QIAOLING ZHANG, FUQUAN LI, LIXIAN LIU, RUI WANG, WEIZHEN LI, YUE ZHAO, JINLONG HUO, HENG XIAO

Abstract: Arrestin-domain-containing proteins belong to the arrestin family, embers of which are important for regulating signal transduction within cells. In this study, two ARRDC genes (ARRDC1 and ARRDC5) of Diannan small-ear pigs were amplified by reverse transcription polymerase chain reaction and cloned for the first time (accession nos.: KF589202 and KF589203). The full-length coding sequence of ARRDC1 was 1284 nucleotides in length (molecular weight, 45.6 kDa; pI, 7.61) and encoded 427 amino acids. The ARRDC5CDS was 1014 nucleotides in length (molecular weight, 37.5 kDa; pI, 7.08) and encoded 337 amino acids. Sequence homology analysis revealed that the amino acid sequences of Diannan small-ear pig ARRDC1 had 94.1%, 93.9%, 93.4%, 92.3%, 89.4%, 88.3%, and 84.7% identity with those of Bos taurus, Tursiops truncatus, Felis catus, Canis lupus, Capra hircus, Homo sapiens,and Mus musculus, respectively; Diannan small-ear pig ARRDC5 had 84.9%, 86.9%, 81.2%, 86.9%, 84.3%, 81.2%, and 71.2% identity with the same species, respectively. The phylogenetic analyses based on the amino acid sequences of ARRDC1 and ARRDC5 from 7 species showed that swine have close genetic relationships with Tursiops truncatus. Tissue expression analyses by quantitative PCR revealed that the mRNA expression levels of ARRDC1 and ARRDC5 were different in various tissues of the Diannan small-ear pig. ARRDC1 was highly expressed in the testis, thyroid, submandibular gland, and kidney, while ARRDC5 was onlyexpressed in the testis, thyroid, and thymus. This study will aid investigations regarding the role of porcine ARRDC1 and ARRDC5 in regulating signal transduction within cells.

Keywords: Diannan small-ear pig, arrestin domain containing 1, arrestin domain containing 5, arrestin family, tissue expression profile

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