In Vivo and in Vitro Micrografting of Pistachio, Pistacia vera L. cv. "Siirt"

Authors: AHMET ONAY, VEDAT PİRİNÇ, FİLİZ ADIYAMAN, ÇİĞDEM IŞIKALAN, ENGİN TİLKAT, DAVUT BAŞARAN

Abstract: In this study, the success of in vivo and in vitro micrografts of pistachio (Pistacia vera L. cv. "Siirt") materials are presented. The only variable tested was age (1, 5, 10, and 30-year-old trees). Ten- to 12-day-old axenic seedlings germinated in vitro or seedlings (3 to 5 months-old) grown in pots in vivo were used as rootstocks. Shoot tips collected from the four age classes of mature trees of pistachio were the source of scions. Firm contact between the scion and rootstock was assured through the use of parafilm tape at the graft junction for in vivo micrografts. The in vivo micrografting system provided good growth and development for new axillary shoots. These plantlets were successfully transplanted and no problems were encountered with the establishment of micrografted plants in soil. The recovery of microscions was slow, but the use of micrografts onto herbaceous rootstocks proved a useful technique.

Keywords: Micrografting, Pistacia vera L., Vegetative propagation

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